Limitations:

Limitations: 

    1)      Reversed phase HPLC‘s accessories are very expensive and it requires high maintenance.

     2)     The technique is pretty time consuming.

    3)      There is no 1 detector that could analyze all substances. 

    4)      Many processing work needs to be done before our samples can be analysed.

Advantages:

1)      Water will not deactivate our stationary phase.

2) It is used for more than 90% of samples in the world.

3) Its uses solvent gradient which can be can be used to:

- improve peak shape and resolution

- prevent solvent wastage

- improve retention times of later peaks

4) It allows the variables such as concentration, pH, temperature and organic solvent type to be controlled.

http://www.angelo.edu/faculty/kboudrea/molecule_gallery/04_aromatics/benzoic_acid_01.gif

method validation: